• Use the Bento Lab and this protocol to extract DNA from your salvia.
• Understand and simplify the protocol.
• Highlight and explain any terms/actions that are new to you.

Date of experiment: April 28, 2022

ref:
Bento: DNA Extraction from Salvia by Georg
https://trembl.notion.site/Bento-DNA-Extraction-from-Salvia-36710c3e07154990b0b274dc06acf469

DNA Extraction from Saliva - bentolab official
https://bento.bio/protocol/biotechnology-101/dna-extraction-from-saliva/

Protocol Notes

//First prepare paper cups for salt, water, saliva, and trash, respectively.

1. Label 1.5m Eppendorf Tube with your name

2. Prepare Saline (=Salt) solution
   Use ca. 5ml of water, mix with a pinch of salt
   // Just put about 5mm of water in the bottom of a paper cup and add a pinch of salt.

3. Rinse your mouth // Around 1 min.

4. Transfer to 1.5ml Eppendorf Tube.
   If you use a Paper Cup, bend it and fill the tube
   // No need to use pipette

5. Centrifuge at 4KG for 90sec // Note that bentolab’s UI is tricky. To change the setting, you must hold down the button and turn it.
   
   ↑ When centrifuging, the tubes should be placed diagonally and the tube lids should be oriented as in the figure.

6. Recover the Pellet by decanting the Supernatant
   The Pellet is the white concentration of cells on the bottom of the tube. The liquid above is the Supernatant
7. Resuspend the Pellet
   Mix the Pellet with the remaining Supernatant by repeatedly flipping and tapping it.
8. Transfer the sample to a 0.2ml PCR tube.
   You can use a 2-20 or 10-100 Pipette.

9. Label the tube.
   // It is helpful to write your name on both the top of the lid and on the side of the tube.

10. Heat the Sample at 99ºC for 10min
11. Mix the Sample by flipping and tapping
12. Centrifuge at 8KG for 90sec
    Use the Adapter Eppendorf tube to fit the PCR tubes in the Centrifuge
    The DNA is now in the Supernatant, NOT in the Pellet
13. Transfer the Supernatant is a new PCR Tube
    Use a 20uL Pipette to transfer the Supernatant - and only the Supernatant - to a new PCR Tube
14. Label and Freeze at -20ºC