2022/03/07 Mon
note
17:30- Homework Peer Review
18:30-19:30 Lecture: Lab hardware Introduction Kas Houthuijs
18:35- Explanation of Hardware
・wetlab hardware
・usables
・wetlab biosafety design
・DIY vs Industrial hardware
・Goals
Particle separation
Particle mixing
Measureing
It is important to precise as much as possible (make everything as measurable as possible)
Control environment
→It’s not that different from a kitchen.
frige（冷蔵庫）, measuring cups(計測カップ), oven(オーブン), pressure cooker(圧力鍋)、slacentrifuge(ぶんぶんチョッパー) etc.
Machine - Goal
Incubator - Control env
Centrifuge - Particle sep
Sterile Hood (clean bench | Fume hood) - Control env
Autoclave - Control env
Shakers and Stirrers - Particle mix
Balance - Measuring
Pipette - Measuring
Microscope - Measuring
Freezer - Control env
Gel Electrophoresis machine - Particle sep
Thermocycler (pcr) - Control env
spectrophotometer - Measuring
Bioreactor - Control env
sequencer - Measuring
high-performance liquid chromatography(HPLC) - Measuring
vortex - Particle mixing
microscope - Measuring
cf）nano drop
・You have to be careful where you use MDF or acrylic for DIY lab equipment, considering ease of cleaning and such.
・Sterile (無菌状態？) conditions can also be made using convection currents created by a burner.
・Autoclave
Tools to kill living organisms at high temperatures and high pressure.
Alcohol, UV light, bleach (bleach) have the same function
After autoclaving, you can throw it away as normal. Tools to kill living organisms at high temperatures due to high pressure
Alcohol, UV light, bleach (bleach) have the same function
After autoclaving, you can usually throw it away (this may not apply if the biosafety level is 2 or higher)
・Microscopes don’t play such an important role in the wet lab.
20:00- つづき
・Spectrophotometer
Determine the concentration of solute in solution
・Bioreactor
bio fuel etc.
Like an aquarium with an incubator or something together.
・sequencer
Measure the amount of charge in the AGCT.
Nanopore measures pH value.
Usables
Eppendolf tubes（エッペンチューブ）
The little ones are used for pcr, etc. Use gloves when handling DNA.
Glass containers
cf) tissue culture flask
Petri dish（シャーレ（German））
Pipettes
Ttips
Inoculation loop（白金耳（Hakkinji））
What’s wet lab?
“A wet lab, or experimental lab, is a type of laboratory where it is necessary to handle various types of chemicals and potential “wet” hazards, so the room has to be carefully designed, constructed, and controlled to avoid spillage and contamination”
Biosafety Design
Permanent structure (advised 掃除しやすいのが大事)
All surfaces are easily cleanable and non absorbent
Autoclave is necessary
Possibly to disinfect hands
Work clothing
Doors and windows closed
No other plants or animals
DIY wet lab hardware can be dangerous or unusable depending on the item.
Second hand is also an option.
22:45- Discussion: What is a Lab? Kas & Maarten
・Empirical cycle（実証研究）
→Evaluation→Observation→Induction→Deduction→Testing→
p value(p値) etc.
Protocols for scientific experiments do not need to be strictly followed if you are doing them on your own, but they become important (especially for reproducibility) when you publish or share them.
The textile lab at waag also tries to document as precisely as possible the procedures used to create the materials.
※There are possibilities (unexpected discoveries and artistry) that come from making room for error.
← But we need to recreate that, too.
← In some cases, you’d have to keep it a secret so people wouldn’t reproduce it.
patent?
Bioplastics and bioleathers, for example, may be limited in their applications by being described as “plastics” or “leathers”.
← Maybe “lab” isn’t the right word.
← What is the difference between laboratory, studio and workshop? Does “lab” have scientific nuances?
Why can’t (or won’t) artists and designers ignore the terms and styles of science from the start?
← In order to use a tool in the first place, it is better to learn it according to the context of that tool, because it is faster to learn and to see how far you can go with the tool.(?)
What else is needed besides a machine to be called a lab?
← protocol? people?
In the first place, those procedures and protocols themselves become artwork.
Tools and protocols are changing the cognitive style of not only artists but also scientists.
cf.) Laboratory Life
ref
Open Source Licenses by Category https://opensource.org/licenses/category
性(セックス)とはなにか Tankobon Hardcover
https://www.amazon.co.jp/dp/4796702237/
Great British Bake Off
https://thegreatbritishbakeoff.co.uk/
Largest bacterium ever discovered has an unexpectedly complex cell
https://www.science.org/content/article/largest-bacterium-ever-discovered-has-unexpectedly-complex-cells
nanopore
https://store.nanoporetech.com/flongle-starter-pack.html
DIY BIOLOGY(lab design)
http://biologicaldesign.info/diy-biology
Maud Bausier
https://maudbausier.com/biolab-kitchen-list-of-needs.html
Inger Le Gué final project
https://ingeltje.github.io/Final%20Project/
Paper Microscope
https://www.foldscope.com/
↑homework
Hand-powered ultralow-cost paper centrifuge
https://www.nature.com/articles/s41551-016-0009
Manu Prakash - Wikipedia
https://en.wikipedia.org/wiki/Manu_Prakash
DIY NanoDrop
https://hackteria.org/wiki/index.php/DIY_NanoDrop
Digital microfluidics
https://en.wikipedia.org/wiki/Digital_microfluidics
Still
https://en.wikipedia.org/wiki/Still
Mysterie superieure Leeuwenhoek-microscoop na 350 jaar opgelost
https://www.tudelft.nl/2018/tu-delft/mysterie-superieure-leeuwenhoek-microscoop-na-350-jaar-opgelost